Evaluasi Potensi Sediaan Sirup Ekstrak Bunga Telang terhadap Edema Perikardium Embrio Zebrafish Melalui Analisis FishInspector
DOI:
https://doi.org/10.57218/jkj.Vol5.Iss3.3137Kata Kunci:
bunga telang, edema, embrio, kardioprotektif, perikardiumAbstrak
Penyakit kardiovaskular seperti hipertensi arteri pulmonal dapat memicu beban kerja berlebih pada ventrikel kanan yang bermanifestasi klinis sebagai edema perifer. Eksplorasi bahan herbal seperti bunga telang (Clitoria ternatea L.) menjadi terobosan preventif karena aktivitas anti-angiogenesis dan kardioprotektifnya. Penelitian ini bertujuan untuk mengevaluasi potensi sediaan sirup ekstrak bunga telang dalam menurunkan derajat edema perikardial pada model embrio zebrafish. Embrio zebrafish usia 24 hours post fertilization dipapar sediaan sirup bunga telang dengan variasi konsentrasi (1,56; 3,12; 6,25; 12,5; 25; dan 50 µg/mL), kontrol negatif (Media E3), dan kontrol positif (CHX 0,1%) selama 96 jam dengan 3x replikasi. Parameter luas area perikardium dianalisis secara kuantitatif menggunakan perangkat lunak FishInspector dan dikomputasi menggunakan Shoelace Formula pada RStudio, dilanjutkan dengan uji statistik menggunakan SPSS. Hasil penelitian menunjukkan luas area perikardium Media E3 sebesar 3.589,91 ± 47,31 pixel² dan CHX 0,1% sebesar 8.327,56 ± 71,97pixel². Luas area perikardium kelompok sirup bunga telang sebesar 7.595,19 ± 87,40 pixel² (50 µg/mL), 5.217,64 ± 65,67 pixel² (25 µg/mL), 3.890,74 ± 50,01 pixel² (12,5 µg/mL), dan 3.699,33 ± 53,98 pixel² (6,25 µg/mL). Kontrol positif CHX 0,1% menunjukkan edema perikardial yang parah dibandingkan Media E3. Pada konsentrasi 25–50 µg/mL, luas area perikardium meningkat, sedangkan konsentrasi 1,56–12,5 µg/mL menunjukkan morfologi perikardium yang secara deskriptif relatif mendekati kontrol negatif Media E3. Namun, hasil One-Way ANOVA menunjukkan tidak terdapat perbedaan signifikan antar kelompok (p = 0,448). Dengan demikian, sirup bunga telang 5% pada konsentrasi 1,56–12,5 µg/mL menunjukkan potensi mempertahankan morfologi perikardium dan tidak menunjukkan efek kardiotoksik berdasarkan parameter yang diamati.
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